Speciation and Characterization of Mycobacterium Tuberculosis Complex Isolated from Multi-Drug Resistance Tuberculosis Patients in Southeastern Nigeria

http://www.doi.org/10.60787/tnhj-707

Authors

Keywords:

Mycobacterium tuberculosis complex, species, MDR-TB and Diagnosis, Nigeria

Abstract

Abstract

In Nigeria and other low-and-middle-income countries, majority of clinical laboratories use Gene Xpert MTB/RIF assay, Hain lifescience MTBDRplus and MTBDRsl for rapid diagnosis of both TB and MDR-TB. These tools diagnose TB disease caused by members of Mycobacterium tuberculosis complex (MTBC), but do not differentiate among members of the MTBC to the species level. Knowledge of the circulating strains of MTBC in a population is critical for effective TB control measures. In this study, 96 MDR-TB strains isolated from patients in Southeast Nigeria were differentiated to species level using Geno Type MTBC kit. Of the 96, 58.30% (n=56) were M. tuberculosis/M. canettii, 11.45% (n=11) were M. africanum and 2.10% (n=2) were M. bovis. However, 11.45% (n=11) were high gram-positive bacteria, 14.60% (n=14) were invalid, while 2.10% (n=2) had no evaluable pattern. Our findings shows that M. tuberculosis/M. canettii and M. africanum are the leading cause of MDR-TB in Southeast Nigeria. Our findings show that M tuberculosis/M. canettii and M. africanum are the leading causes of MDR-TB amongst the study population. Furthermore, the results of this study indicate that M. bovis remains an important cause of tuberculosis particularly amongst groups at risk.

References

Asare P, Asante-Poku A, Osei-Wusu S, Otchere ID, Yeboah-Manu D. The Relevance of Genomic Epidemiology for Control of Tuberculosis in West Africa. Front Public Heal. 2021;9:1–17.

Sylverken AA, Kwarteng A, Twumasi-Ankrah S, Owusu M, Arthur RA, Dumevi RM, et al. The burden of drug resistance tuberculosis in Ghana; results of the First National Survey. PLoS One [Internet]. 2021;16(6 June):1–14. Available from: http://dx.doi.org/10.1371/journal.pone.0252819

Miller MA, Buss P, Roos EO, Hausler G, Dippenaar A, Mitchell E, et al. Fatal tuberculosis in a free-ranging African elephant and one health implications of human pathogens in wildlife. Front Vet Sci. 2019; 6:1–8.

Tomeny EM, Nightingale R, Chinoko B, Nikolaidis GF, Madan JJ, Worrall E, et al. TB morbidity estimates overlook the contribution of post-TB disability: Evidence from urban Malawi. BMJ Glob Heal. 2022;7(5):1–12.

Živanović I, Vuković D, Dakić I, Savić B. Species of mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens from Serbia. Arch Biol Sci. 2014;66(2):553–61.

Brites D, Loiseau C, Menardo F, Borrell S, Boniotti MB, Warren R, et al. A New Phylogenetic Framework for the Animal-Adapted Mycobacterium tuberculosis Complex. 2018;9 :1–14.

Federal Ministral of Health (FMoH). Department of Public Health. National Tuberculosis and Leprosy Control Programme. National Guidelines for Culture and Drug Susceptibility Testing.

Abuja, Nigeria, December 2015. Available from https://ntblcp.org.ng/resources/national-guidelines-for-culture-and-drug-susceptibility-testing/

Cá B, Fonseca KL, Sousa J, Maceiras AR, Machado D, Sanca L, et al. Experimental Evidence for Limited in vivo Virulence of Mycobacterium africanum. Front Microbiol. 2019;10 :1–9.

Blouin Y, Cazajous G, Dehan C, Soler C, Vong R, Hassan MO, et al. Progenitor “Mycobacterium canettii” Clone responsible for lymph node tuberculosis epidemic, Djibouti. Emerg Infect Dis. 2014;20(1):21–8.

Bakuła Z, Wuyep VB, Bartocha Ł, Vyazovaya A, Ikeh EI, Bielecki J, et al. Molecular snapshot of drug-resistant Mycobacterium tuberculosis strains from the Plateau State, Nigeria. PLoS One [Internet]. 2022;17(5 May):1–13. Available from: http://dx.doi.org/10.1371/journal.pone.0266837

Chuku A, Ajide B, Lawson L, Orule K, Adogo L. Differentiation and Characterization of Mycobacterium tuberculosis Complex Causing Pulmonary Tuberculosis across North Central Nigeria. Int J Trop Dis Heal. 2019 :1–10.

Osei-wusu S, Otchere ID, Asare P, Ntoumi F, Zumla A, Asante-poku A, et al. International Journal of Infectious Diseases Relevance of genomic diversity of Mycobacterium tuberculosis complex in Africa. Int J Infect Dis [Internet]. 2022;124:S47–9. Available from: https://doi.org/10.1016/j.ijid.2022.03.016

Asante-poku A, Otchere ID, Osei-wusu S, Sarpong E, Baddoo A, Forson A, et al. Molecular epidemiology of Mycobacterium africanum in Ghana. BMC Infect Dis [Internet]. 2016;1–8. Available from: http://dx.doi.org/10.1186/s12879-016-1725-6

Ofori-anyinam B, Kanuteh F, Agbla SC. Impact of the Mycobaterium africanum West Africa 2 Lineage on TB Diagnostics in West Africa : Decreased Sensitivity of Rapid Identification Tests in The Gambia. 2016;1–12.

Adesokan HK, Jenkins AO, Soolingen D Van, Cadmus SIB. Mycobacterium bovis infection in livestock workers in Ibadan , Nigeria : evidence of occupational exposure. 2012;16:1388–92.

Sa'idu AS, Okolocha EC, Dzikwi AA, Gamawa AA, Ibrahim S, Kwaga JKP, et al. Public Health Implications and Risk Factors Assessment of Mycobacterium bovis Infections among Abattoir Personnel in Bauchi State, Nigeria. Journal of Veterinary Medicine 2015; Vol. 2015. Article ID 718193. Available from https://doi.org/10.1155/2015/718193

Downloads

Published

2024-01-15

How to Cite

Gweba, C., Ojule, I. N., Panwal, M. T., Girei, R. S., & Mamman, K. E. (2024). Speciation and Characterization of Mycobacterium Tuberculosis Complex Isolated from Multi-Drug Resistance Tuberculosis Patients in Southeastern Nigeria: http://www.doi.org/10.60787/tnhj-707. The Nigerian Health Journal, 23(4), 874–879. Retrieved from https://tnhjph.com/index.php/tnhj/article/view/707

Issue

Section

Original Articles

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.