Bacterial Isolation, Identification and Antibiogram of Frequently Used Fomite in a Tertiary Hospital in Ado-Ekiti, Ekiti State, Nigeria
DOI:
https://doi.org/10.60787/tnhj.v24i4.934Keywords:
Nosocomial infection, fomites, healthcare facility, antibiogramAbstract
Background: Nosocomial infections or hospital-acquired infections (HAI) are infections which present within 48 hours of hospital admission, within three days of discharge, or postoperatively within a month. These infections are responsible for increased morbidity and mortality and treatment expenses due to extended stays in hospitals. This study identifies pathogens on fomites in a Multi-System Hospital, Ado-Ekiti, Ekiti State.
Method: A total of 90 fomite samples, were collected from various sources within the hospital using swab sticks and sent to the lab for the purpose of identifying the infectious agents. After being inoculated on MacConkey, blood, sabouraud, and chocolate agar, the samples were incubated for 18 to 24 hours at 37 °C.
Results: The prevalence rate of 47.8% was recorded in this study. Microbiology laboratory and accident and emergency units had the most contamination with 8.9% respectively while theatre unit and general outpatient department had the least contamination with 4.4% respectively. The door/drawer and fridge handles had the highest contamination rate of 16.7%, 3.3% were from mouse/keyboard, 7.8% were from sink, 6.7% were from tap handle, 2.2% were from safety cabinet, 2.2% were from work bench, 4.4% were from patients’ bed, 3.3% were from oxygen tank and operating light respectively, while blood cell counter had the least contamination rate of 1.1%.
Conclusion: This study has revealed that inanimate surfaces from the hospital harbour member Enterobacteriaceae. It establishes the fact that Nosocomial infections still persist within our healthcare setting and proves that fomites are possible vehicles of transmission within healthcare facilities.
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Chahardehi AM, Ghasemi AH: Improving management of hospital-acquired infections in the healthcare setting using antimicrobial coating. Life Sci. 2023, 1:23-39. 10.22034/LSSJ.2023.1.56
Singh, Dr.S., Aruna, Dr., kumari, G. and Priya, P. (2023). Hospital Acquired Infections during Patient Incubation at Tertiary Care Hospital of Northern India. International Journal of Medical Science and Clinical Research Studies, 03(03). doi:https://doi.org/10.47191/ijmscrs/v3-i3-24.
Goyal, M. and Chaudhry, D. (2019). Impact of Educational and Training Programs on Knowledge of Healthcare Students Regarding Nosocomial Infections, Standard Precautions and Hand Hygiene: A Study at Tertiary Care Hospital. Indian journal of critical care medicine: peer-reviewed, official publication of Indian Society of Critical Care Medicine, [online] 23(5), pp.227–231. doi:https://doi.org/10.5005/jp-journals-10071-23166.
Alemu, W., Belachew, T. & Yimam, Y. Healthcare-associated infections and their determinants among patients admitted to general hospitals in Ethiopia: A systematic review and meta-analysis. BMC Infect. Dis. 20(1), 178 (2020).
Nwankwo, E. O. Epidemiology of healthcare-associated infections in Nigeria: A systematic review and meta-analysis. BMJ Open. 8(6), e020431 (2018).
Mythri, H. and Kashinath, K. (2014). Nosocomial infections in patients admitted in intensive care unit of a Tertiary Health Center, India. Annals of Medical and Health Sciences Research, 4(5), p.738. doi:https://doi.org/10.4103/2141-9248.141540.
Haque, M., Sartelli, M., McKimm, J. and Abu Bakar, M.B. (2019). Health care-associated Infections – an Overview. Infection and Drug Resistance, Volume 11(11), pp.2321–2333. doi:https://doi.org/10.2147/IDR.S177247.
Monegro AF, Muppidi V, Regunath H (2020) Hospital acquired infections. In StatPearls (Internet), StatPearls Publishing. Available online: http://www.ncbi.nlm.nih.gov/books/NBK44 1857 (Accessed 20 February 2024).
Sikora A, Zahra F (2021) Nosocomial infections. In StatPearls (Internet), StatPearls Publishing: http://www.ncbi.nlm.nih.gov/books/ NBK559312 (Accessed 13 February 2024).
Murray, C.J. (2022). Global Burden of Bacterial Antimicrobial Resistance in 2019: A Systematic Analysis. The Lancet, [online] 399(10325), pp.629–655. doi:https://doi.org/10.1016/S0140-6736(21)02724-0.
Kiros, T., Damtie, S., Eyayu, T., Tiruneh, T., Hailemichael, W. and Workineh, L. (2021). Bacterial Pathogens and Their Antimicrobial Resistance Patterns of Inanimate Surfaces and Equipment in Ethiopia: A Systematic Review and Meta-analysis. BioMed Research International, 2021(doi: 10.1155/2021/5519847.), pp.1–25. doi:https://doi.org/10.1155/2021/5519847.
Jabłońska-Trypuć, A., Makuła, M., Włodarczyk-Makuła, M., Wołejko, E., Wydro, U., Serra-Majem, L. and Wiater, J. (2022). Inanimate Surfaces as a Source of Hospital Infections Caused by Fungi, Bacteria and Viruses with Particular Emphasis on SARS-CoV-2. International Journal of Environmental Research and Public Health, 19(13), p.8121. doi:https://doi.org/10.3390/ijerph19138121.
Barrie, D., Hoffman, P.N., Wilson, J.A. and Kramer, J.M. (1994). Contamination of hospital linen by Bacillus cereus. Epidemiology and Infection, 113(2), pp.297–306. doi:https://doi.org/10.1017/s0950268800051724.
Osterholm MT, Hederg CW, MacDonald K. Epidemiology of infectious diseases. In: Mandell, Douglas and Bennett’s principles and practice of infectious diseases. The Lancet Infectious Diseases. 2005;5(7):405-6.
Nwankiti O.O, Ndako J.A, Nwankiti A.J, Okeke O.I, Uzoechina A.R, Agada, G.O. Computer Keyboard and Mouse: Etiologic Agents for Microbial Infections. Nat Sci 2012;10(10):162-166]. (ISSN: 1545-0740). http://www.sciencepub.net/nature. 23
Umar, D., Basheer, B., Husain, A., Baroudi, K., Ahamed, F. and Kumar, A. (2015). Evaluation of bacterial contamination in a clinical environment. PubMed, 7(1), pp.53–5.
Gugliotta, C., G. Deiana, Dettori, M., Sotgiu, G., Azara, A. and Castiglia, P. (2020). Prevalence study on healthcare associated infections and on the use of antimicrobials carried out with the light protocol of the European Centre for Disease Prevention and Control. doi: 10.7416/ai.2020.2359., 32(4), pp.357–367. doi:https://doi.org/10.7416/ai.2020.2359.
Gozel, M.G., Hekimoglu, C.H., Gozel, E.Y., Batir, E., McLaws, M.-L. and Mese, E.A. (2021). National Infection Control Program in Turkey: The healthcare associated infection rate experiences over 10 years. American Journal of Infection Control, 49(7), pp.885–892. doi:https://doi.org/10.1016/j.ajic.2020.12.013.
Owolabi, j. t. (2020). Geographical Analysis of Green Belt on Urban Climate of Ado-Ekiti. American Journal of Research Communication,
(9), 1–14
Cochran, W. G., Mosteller, F., and Tukcy, J. W. (1954). Statistical Problems of the Kinsey Report. American Statistical Association, Washington, D.C., p. 280
Garba, Muhammad Rayyan; Gadanya, Muktar Ahmed1; Iliyasu, Zubairu1; Gajida, Auwal Umar1. Comparative Study of Patients' Satisfaction between National Health Insurance Scheme-Insured and Un-Insured Patients Attending a Northern Nigerian Tertiary Hospital. Nigerian Journal of Basic and Clinical Sciences 15(2): 118-126, Jul–Dec 2018. | DOI: 10.4103/njbcs.njbcs_48_16
Sepideh Bagheri Nejad, Benedetta Allegranzi, Shamsuzzoha B Syed, Benjamin Ellis, Didier Pittet. (2011) Health-care-associated infection in Africa: a systematic review. Bull World Health Organ. (2011); 89:757–765
Abubakar, U., Amir, O., & Rodríguez-Baño, J. (2022). Healthcare-associated infections in Africa: A systematic review and meta-analysis of point prevalence studies. Journal of Pharmaceutical Policy and Practice, 15, 99.
Muhammad, I., Ismail, W., Samsuddin, N. and Alias, N. (2023). Pathogens from fomites in clinical setting: a scoping review. IIUM Journal of Orofac Health Science, 4(1), 59-79
Fasugba, O., Gardner, A., Mitchell, B.G. and Mnatzaganian, G. (2020). Ciprofloxacin resistance in community- and hospitalacquired escherichia coli urinary tract infections: a systematic review and meta-analysis of observational studies. BMC Infect Disease, 15(5), 545-550.
Dawodu, O.G. and Akanbi, R.B. (2021). Isolation and identification of microorganisms associated with automated teller machines on Federal Polytechnic Ede campus. PLoS One, 16(8), 658-662
Iduh, U.M., Chollom, C.S. and Nuhu, A. (2020). Nosocomial infections in post-operative wounds due to Staphylococcus aureus and Pseudomonas aeruginosa in Benue State Nigeria. African Journal of Microbiology Research, 9(36), 1989-1996.
Chen, K.-H., Chen, L.-R. and Wang, Y.-K. (2014). Contamination of Medical Charts: An Important Source of Potential Infection in Hospitals. PLoS ONE, 9(2), p.e78512. doi:https://doi.org/10.1371/journal.pone.0078512.
Randle, J., Arthur, A. and Vaughan, N. (2010). Twenty-four-hour observational study of hospital hand hygiene compliance. Journal of Hospital Infection, 76(3), pp.252–255. doi:https://doi.org/10.1016/j.jhin.2010.06.027.
Rothe, C., Schlaich, C. and Thompson, S. (2013). Healthcare-associated infections in sub-Saharan Africa. Journal of Hospital Infection, 85(4), pp.257–267. doi:https://doi.org/10.1016/j.jhin.2013.09.008.
Birru, M., Mengistu, M. and Siraj, M. (2021). Magnitude, diversity, and antibiograms of bacteria isolated from patient-care equipment and inanimate objects of selected wards in Arba Minch General Hospital, Southern Ethiopia. Research Report in Tropical Medicine, 12(2), 39-49.
Suleyman, G., Alangaden, G. and Bardossy, A.C. (2018). The role of environmental
contamination in the transmission of nosocomial pathogens and healthcare-associated infections. Current Infectious Disease Report, 20(6), 12-18.
Irek, E.O. (2018). A systematic review of healthcare-associated infections in Africa: An antimicrobial resistance perspective. African Journal of Laboratory Medicine, 7(2), 1–9.
Yallew, W.W., Kumie A. and Yehuala F.M. (2019). Point prevalence of hospital-acquired infections in two teaching hospitals of Amhara region in Ethiopia. Drug, Healthcare and Patient Safety, 8(2), 71-75
De-Alwis, W.R., Pakirisamy, P., Wai, S.L. and Xiaofen, E.C. (2022). A study on hand contamination and hand washing practices among medical students. ISRN Public Health, 12(2), 483-492.
Itah, A.Y. and Ben, A.E. (2020). Incidence of enteric bacteria and staphylococcus aureus in day care centers in Akwa Ibom State, Nigeria. Southeast Asian Journal of Tropical Medicine Public Health, 35(1), 202-209.
Petignat, C., Francioli, P. and Nahimana, N. (2021). Exogenous source of Pseudomonas aeruginosa in an Intensive Care Unit patient: Implementation of infection control and follow-up with molecular typing. Infection Control Hospital Epidemiology, 27(9), 953-957.
Saka, K.H., Akanbi, A.A. and Obasa, T.O. (2019). Pathogenic aerobic bacterial contaminants on non-critical hospital surfaces within Paediatric Ward of a Nigerian Hospital. Journal of Medical Microbiology & Diagnosis, 5(241), 2161-2703.
Ayalew, W., Mulu, W. and Biadglegne, F. (2019). Bacterial contamination and antibiogram of isolates from health care workers’ fomites at Felege Hiwot Referral Hospital, Northwest Ethiopia. Ethiopian Journal of Health Development, 32(2), 128-141
Breijyeh, Z., Jubeh, B. and Karaman, R. (2020). Resistance of Gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules, 25(6), 1340-147.
Recalde-Reyes, D.P., Alfonso-Ortiz, N. and Fuentes-Quimbayo, M.F. (2021). Genotypic and phenotypic resistance profile present in bacteria isolated from fomites in Armenia, Quindío Colombia period June-July 2019. Infection, 25(1), 22-27.
Kraemer, S.A., Ramachandran, A. and Perron, G.G. (2019). Antibiotic pollution in the environment: From microbial ecology to public policy. Microorganisms, 7(6), 180-187.
Maryam, A., Hadiza, U.S. and Aminu, U.M. (2020). Characterization and determination of antibiotic susceptibility pattern of bacteria isolated from some fomites in a teaching hospital in northern Nigeria. African Journal of Microbiology Research, 8(8), 814-818.
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