Comparative Effects of L-Carnitine and T-Bhq on Reproductive Hormone Dysregulation in Alcohol-Exposed Wistar Rats
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Abstract
Background: Reproductive dysfunction has been linked to chronic alcoholism, especially due to oxidative stress. Antioxidants like L-carnitine and tert-butylhydroquinone (tBHQ) could mitigate alcohol-induced reproductive toxicity, but their comparative effects are understudied. This study compared the effects of l-carnitine and t-BHQ on reproductive hormone dysregulation in alcohol-exposed Wistar rats.
Methodology: Thirty-five male Wistar rats were divided into seven groups (n=5): control, alcohol-only, alcohol+L-carnitine, alcohol+tBHQ, alcohol+L-carnitine+tBHQ, L-carnitine-only, and tBHQ-only. Alcohol was administered orally (2 g/kg/day), L-carnitine (100 mg/kg/day), and tBHQ (50 mg/kg/day) for 60 days. At the end of the experimental period, serum testosterone, dihydrotestosterone (DHT), 5-α reductase, luteinizing hormone (LH), and follicle stimulating hormone (FSH) were assayed.
Results: Alcohol exposure significantly reduced testosterone (from 10.33 ng/ mL in the control group to 1.12 ng/mL) while significantly increasing the levels of DHT (59.67 pg/Ml to 1237.67 pg/mL), 5-α reductase (91.83 pg/mL to 698.33 pg/mL), LH (26.83 mIU/mL to 64.50 mIU/mL) and FSH (2.98 mIU/mL to 18.33 mIU/mL). L-carnitine and tBHQ independently restored testosterone to above-control levels (16.67 and 17.33 ng/mL, respectively), with their co-administration yielded much more increase. Both agents significantly reduced alcohol-induced increase in DHT and 5-α reductase, with tBHQ exerting a stronger suppressive effect. Similarly, LH and FSH concentrations were normalized toward control values following treatment.
Conclusion: Results shows that L-carnitine and tBHQ mitigated hormone dysregulation in male Wistar rats caused by excessive alcohol exposure. These findings suggest that L-carnitine preserves mitochondrial integrity to sustain steroidogenesis, while tBHQ more effectively modulates enzyme activity and oxidative stress in the testes.
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References
1.Park SH, Kim DJ. Global and regional impacts of alcohol use on public health: Emphasis on alcohol policies. Clinical and molecular hepatology. 2020 Oct 1;26(4):652.
2.Mehta G, Sheron N. No safe level of alcohol consumption–Implications for global health. Journal of hepatology. 2019 Apr 1;70(4):587-9.
3.World Health Organization. Global status report on alcohol and health 2018. World Health Organization; 2018.
4.Genchi VA, Cignarelli A, Sansone A, Yannas D, Dalla Valentina L, Renda Livraghi D, Spaggiari G, Santi D. Understanding the role of alcohol in metabolic dysfunction and male infertility. Metabolites. 2024 Nov 15;14(11):626.
5.Sansone A, Di Dato C, de Angelis C, Menafra D, Pozza C, Pivonello R, Isidori A, Gianfrilli D. Smoke, alcohol and drug addiction and male fertility. Reproductive biology and endocrinology. 2018 Jan 15;16(1):3.
6.Rotimi DE, Acho MA, Falana BM, Olaolu TD, Mgbojikwe I, Ojo OA, Adeyemi OS. Oxidative stress-induced hormonal disruption in male reproduction. Reproductive Sciences. 2024 Oct;31(10):2943-56.
7.Rachdaoui N, Sarkar DK. Pathophysiology of the effects of alcohol abuse on the endocrine system. Alcohol research: current reviews. 2017;38(2):255.
8.Duca Y, Aversa A, Condorelli RA, Calogero AE, La Vignera S. Substance abuse and male hypogonadism. Journal of clinical medicine. 2019 May 22;8(5):732.
9.Siervo GE, Vieira HR, Ogo FM, Fernandez CD, Gonçalves GD, Mesquita SF, Anselmo-Franci JA, Cecchini R, Guarnier FA, Fernandes GS. Spermatic and testicular damages in rats exposed to ethanol: influence of lipid peroxidation but not testosterone. Toxicology. 2015 Apr 1;330:1-8.
10.Darbandi M, Darbandi S, Agarwal A, Sengupta P, Durairajanayagam D, Henkel R, Sadeghi MR. Reactive oxygen species and male reproductive hormones. Reproductive Biology and Endocrinology. 2018 Sep 11;16(1):87.
11.Monageng E, Offor U, Takalani NB, Mohlala K, Opuwari CS. A review on the impact of oxidative stress and medicinal plants on Leydig cells. Antioxidants. 2023 Aug 4;12(8):1559.
12.Chimento A, De Luca A, Venditti M, De Amicis F, Pezzi V. Beneficial Effects of Resveratrol on Testicular Functions: Focus on Its Antioxidant Properties. Cells. 2025 Jul 21;14(14):1122.
13.Ketchem JM, Bowman EJ, Isales CM. Male sex hormones, aging, and inflammation. Biogerontology. 2023 Feb;24(1):1-25.
14.Das PK, Mukherjee J, Banerjee D. Functional morphology of the male reproductive system. InTextbook of veterinary physiology 2023 Sep 1 (pp. 441-476). Singapore: Springer Nature Singapore.
15.Oduwole OO, Huhtaniemi IT, Misrahi M. The roles of luteinizing hormone, follicle-stimulating hormone and testosterone in spermatogenesis and folliculogenesis revisited. International journal of molecular sciences. 2021 Nov 25;22(23):12735.
16.Cohen J, Nassau DE, Patel P, Ramasamy R. Low testosterone in adolescents & young adults. Frontiers in endocrinology. 2020 Jan 10;10:916.
17.Virmani MA, Cirulli M. The role of l-carnitine in mitochondria, prevention of metabolic inflexibility and disease initiation. International journal of molecular sciences. 2022 Feb 28;23(5):2717.
18.Roseiro LC, Santos C. Carnitines (including l-carnitine, acetyl-carnitine, and proprionyl-carnitine). InNonvitamin and nonmineral nutritional supplements 2019 Jan 1 (pp. 45-52). Academic Press.
19.Liu X, Yang L, Zhang G, Ling J. Neuroprotective effects of phenolic antioxidant tert-butylhydroquinone (tBHQ) in brain diseases. Molecular Neurobiology. 2023 Sep;60(9):4909-23.
20.Khezerlou A, pouya Akhlaghi A, Alizadeh AM, Dehghan P, Maleki P. Alarming impact of the excessive use of tert-butylhydroquinone in food products: A narrative review. Toxicology reports. 2022 Jan 1;9:1066-75.
21.Nna VU, Ujah GA, Suleiman JB, Mohamed M, Nwokocha C, Akpan TJ, Ekuma HC, Fubara VV, Kekung-Asu CB, Osim EE. Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis. Toxicology. 2020 Aug 1;441:152528.
22.Ujah GA, Nna VU, Suleiman JB, Eleazu C, Nwokocha C, Rebene JA, Imowo MU, Obi EO, Amachree C, Udechukwu EC, Mohamed M. Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes and improves spermatogenesis in rats. Scientific Reports. 2021 Mar 9;11(1):5522.
23.Oremosu AA, Akang EN. Impact of alcohol on male reproductive hormones, oxidative stress and semen parameters in Sprague–Dawley rats. Middle East Fertility Society Journal. 2015 Jun 1;20(2):114-8.
24.Koohpeyma F, Gholizadeh F, Hafezi H, Hajiaghayi M, Siri M, Allahyari S, Maleki MH, Asmarian N, Bayat E, Dastghaib S. The protective effect of L-carnitine on testosterone synthesis pathway, and spermatogenesis in monosodium glutamate-induced rats. BMC complementary medicine and therapies. 2022 Oct 13;22(1):269.
25.Chen Y, Zhang X, Lan S, Liang S, Zhang M, Zhang S, Liu Y, Li L, Wei H, Zhang S. Tert-Butylhydroquinone Mitigates T-2-Toxin-Induced Testicular Dysfunction by Targeting Oxidative Stress, Inflammation, and Apoptosis in Rats. Toxics. 2024 May 5;12(5):335.
26.ELK Biotechnology. Testo (Testosterone) ELISA Kit.
www.elkbiotech.com/upload/file/ELISA/ELK10254-1.pdf
27.Eagle Biosciences, Inc. Dihydrotestosterone (DHT) ELISA Assay Kit. https://eaglebio.com/wp-content/uploads/data-pdf/dht31-dht-elisa-assay-kit-packageinsert.pdf
28.Santucci L, Graham TJ, MD DH. Inhibition of testosterone production by rat Leydig cells with ethanol and acetaldehyde: prevention of ethanol toxicity with 4‐methylpyrazole. Alcoholism: Clinical and Experimental Research. 1983 Mar;7(2):135-9.
29.Smith SJ, Lopresti AL, Fairchild TJ. The effects of alcohol on testosterone synthesis in men: a review. Expert review of endocrinology & metabolism. 2023 Mar 4;18(2):155-66.
30.Mateus FG, Moreira S, Martins AD, Oliveira PF, Alves MG, Pereira MD. L-carnitine and male fertility: is supplementation beneficial? Journal of Clinical Medicine. 2023 Sep 6;12(18):5796.
31.Abdel‐Emam RA, Ahmed EA. Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats. Veterinary medicine and science. 2021 Jul;7(4):1426-35.
32.Fajardo A. Novel mechanisms of androgen receptor degradation by alpha-tocopherylquinone and curcumin analog 27.
33.Rubin E, Lieber CS, Altman K, Gordon GG, Southren AL. Prolonged ethanol consumption increases testosterone metabolism in the liver. Science. 1976 Feb 13;191(4227):563-4.
34.Van Thiel DH. Ethyl alcohol and gonadal function. Hospital Practice. 1984 Nov 1;19(11):152-8.
35.Shimon I, Lubina A, Gorfine M, Ilany J. Feedback inhibition of gonadotropins by testosterone in men with hypogonadotropic hypogonadism: comparison to the intact pituitary‐testicular axis in primary hypogonadism. Journal of andrology. 2006 May 6;27(3):358-64.
36.Thiel D, Lester R, Vaitukaitis J. Evidence for a defect in pituitary secretion of luteinizing hormone in chronic alcoholic men. The Journal of Clinical Endocrinology & Metabolism. 1978 Sep 1;47(3):499-507.
37.Castilla-García A, Santolaria-Fernández FJ, González-Reimers CE, Batista-López N, González-García C, Jorge-Hernández J, Hernández-nieto L. Alcohol-induced hypogonadism: reversal after ethanol withdrawal. Drug and alcohol dependence. 1987 Nov 30;20(3):255-60.
38.Salonen I, Huhtaniemi I. Effects of chronic ethanol diet on pituitary-testicular function of the rat. Biology of reproduction. 1990 Jan 1;42(1):55-62.
39.Ogilvie KM, Rivier C. Effect of alcohol on the proestrous surge of luteinizing hormone (LH) and the activation of LH-releasing hormone (LHRH) neurons in the female rat. Journal of Neuroscience. 1997 Apr 1;17(7):2595-604.
40.Finelli R, Mottola F, Agarwal A. Impact of alcohol consumption on male fertility potential: a narrative review. International journal of environmental research and public health. 2021 Dec 29;19(1):328.